mouse cd4 t cell negative selection kit  (MedChemExpress)

Journal: Cell Death Discovery

Article Title: CXCL16 knockout inhibit asthma airway inflammation by suppressing H2-DM molecular mediated antigen presentation

doi: 10.1038/s41420-025-02371-6

Figure Lengend Snippet: A GO enrichment of biological process for downregulated differentially expressed genes in CXCL16-knockout mouse BMDCs versus wild-type mouse BMDCs after Aspergillus challenge ( n = 3). B CD11c + BMDCs from wild-type mice or CXCL16-knockout mice were pulsed with 20 μg OVA 323-339 peptide and incubated at a 1:10 ratio with CFSE-labeled splenic CD4 + OT-II T cells for 4 days. OVA-specific proliferation is presented as a proliferation index ( n = 4). C Phagocytosis of FITC-dextran by CD11c + sorting BMDCs in vitro ( n = 3). D Unsupervised clustering of genes involved in biological processes of antigen processing and presentation, MHC class II biosynthetic process, regulation of phagocytosis, phagocytosis, and recognition were analyzed ( n = 3). E Real-time PCR was performed to detect downregulated differentially expressed PRR genes of Aspergillus -challenged CXCL16-knockout BMDCs and wild-type BMDCs ( n = 3). F The mRNA level of H2-DM on Aspergillus -induced CXCL16-knockout mouse BMDCs or wild-type mouse BMDCs ( n = 6). G The mRNA level of H2-Oa and H2-Ob in CXCL16-knockout BMDCs ( n = 6). * P < 0.05, ** P < 0.01 Aspergillus -challenged CXCL16-knockout mice versus wild-type mice. A.f . stands for Aspergillus .

Article Snippet: Naïve CD4 + T cells were purified from the spleens of OT-II mice by a CD4 + bead negative selection kit (MACS Miltenyi Biotec) and were labeled with 5 μM CFSE (ThermoFisher Scientific) at 37 °C for 30 min.

Techniques: Knock-Out, Incubation, Labeling, In Vitro, Real-time Polymerase Chain Reaction